DAIBIO Drug Toxicity Experimental Results with University and Academy in April 2022

The study monitored the general condition of mice after continuous drug administration during the first 4 hours and 72 hours and then continued until the end of 7 days. The results showed that mice in all groups exhibited normal activity and movement. None of the mice showed signs of motor nerve damage, neurological stimulation, or inhibition. All mice maintained smooth fur without matting or wetness.

Observation of autonomic nervous system effects indicated that the drug did not affect the autonomic nervous system in any group. Examination of the pupils showed that all mice had normal pupil responses, with no signs of constriction or dilation. None of the mice exhibited sweating or dry, red skin.

Respiratory assessment showed that all mice breathed normally, without signs of dyspnea, cyanosis, or any abnormal respiratory conditions. Regarding food and water intake, since the mice consumed a large and continuous amount of the drug on the first day, their eating and drinking habits were evaluated starting from the second day. Observations indicated that all mice in all groups ate and drank normally, with no signs of refusal or increased consumption.

Fecal observations showed that all mice had normal feces, without looseness or discoloration. Daily checks of the anal area revealed it was dry and free of fecal residue. No signs of pain, allergies, or irritation, such as nasal discharge or scratching due to itching, were observed. All mice across all groups showed no abnormal signs.

When the white mice were administered the test drug at varying doses, from the lowest dose of 5.0 g/kg body weight to the highest dose of 17.5 g/kg body weight, the mortality rate for each group was assessed and presented in Table 1.

Table 1. Assessment of mortality rates in each group after 72 hours in the acute toxicity study

The results in Table 2 showed no significant differences in weight gain rates between the control and test groups at the start of the study (N0), after 7 days (N7), 14 days (N14), and 28 days (N28). The weight gain rates of mice were similar across control and test groups at these different time points.

Hematological indices were assessed after 28 days of consuming Ageless Man No6 capsules (Table 3). Comparisons of hematological indices (e.g., white blood cell count, red blood cell count, hemoglobin, hematocrit, platelet count) before treatment (N0), and after 14 (N14) and 28 days (N28) revealed no statistically significant differences (p>0.05) between the control group and the groups consuming doses 1, 2, and 3.

Table 3. Effect of Ageless Man No6 Capsules on Body Weight Gain of Male and Female Mice Over Time

Note: WBC: white blood cells; RBC: red blood cells; HGB: hemoglobin; HCT: hematocrit; PLT: platelets.

Biochemical Indices Assessment

Biochemical indices after 28 days of Ageless Man No6 capsule consumption were shown in Table 4. At the time points before treatment, and 14 and 28 days post-treatment, biochemical markers such as AST and ALT activity, total protein levels, total cholesterol levels, and creatinine concentrations showed no significant differences (p>0.05) between test groups (doses 1, 2, and 3) and the control group.

Table 5. Effect of 28-Day Consumption of Ageless Man No6 Powder on Biochemical Indices of Male and Female Mice