Lectins are proteins that selectively bind to the carbohydrate moieties of glycoproteins. We have previously reported.
Sources: http://www.macb.org.my/wp-content/uploads/2010/04/10th-ASM-2000-Abstract.pdf
FP 1:
THE APPLICATION OF CHAMPEDAK MANNOSE-BINDING LECTIN IN THE DETECTION AND ISOLATION OF SELECTIVE HUMAN ACUTE-PHASE PROTEINS.
Hashim OH, Ahmad F, Shuib AS.
Department of Biochemistry, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur.
Lectins are proteins that selectively bind to the carbohydrate moieties of glycoproteins. We have previously reported
the isolation of a mannose-binding and IgE-and IgM-reactive lectin from the seeds of champedak (Artocarpus integer)
(J. Immunol. Meth. (1997)
209, 177-186), and also demonstrated that the lectin was mitogenic to mouse Tlymphocytes (Immunol. Invest. (1998)
27, 395-404). In this study, we have investigated the interaction of the lectinwith human serum glycoproteins that were resolved by 2-dimensional (2-D) gel electrophoresis. The lectin demonstrated strong interaction with haptoglobin, hemopexin, orosomucoid, α1-antitrypsin,
β2-HS glycoprotein, transferrin, IgA, IgM and IgG of the human serum. With exceptions of the immunoglobulins, all the other
glycoproteins are categorised as acute-phase proteins whose plasma concentrations are substantially altered in
response to inflammation. This novel Western blotting technique, using a plant lectin as probe, may be conveniently
utilised to detect and possibly quantify these acute-phase proteins. The technique is certainly better than
nephelometry or turbidimetry, which are presently used in the determination of acute-phase responses. The accuracy
of these methodologies, which utilises specific antisera, may often be compromised by the presence of excessive
amount of antigen, particles, macromolecules and lipoproteins. On the other hand, the 2-D separated profiles of the
plasma acute-phase proteins, displayed by an enzyme-conjugated lectin probe, can be visualised directly and analysed
simultaneously. Subjecting human serum to immobilised-lectin-M affinity chromatography may also isolate some of
the acute-phase proteins.
FP 2:
A COMPUTER PROGRAM FOR OFFLINE DATA MANAGEMENT IN OLYMPUS AU640
CLINICAL CHEMISTRY ANALYSER
Loh SH
Department of Pathology, Sultanah Aminah Hospital, Johor Bahru, 80100 Malaysia
The Olympus AU640 Clinical Chemistry Analyser allows patients’ test data to be output offline to a floppy diskette.
An Excel program is written to convert test codes into test names and sort the data into a proper database format. The
program is attached to the Microsoft Excel Application Program as an add-in. The program appears as a menu, namely
AU640, in the menu bar when Excel is opened, which contains 4 items: edit_data, print_record, edit_workload,
print_workload. After downloading the daily data, the diskette is inserted into the disk drive of an offline computer.
Clicking the respective item pad will produce and print the final data record and workload. This program saves
considerable time in manually recording the test results. It will be useful for centers where complete Laboratory
Information System cannot be installed yet.
FP3:
POLYMERASE CHAIN REACTION (PCR) APPLICATION FOR EARLY DIAGNOSIS OF
NASOPHARYNGEAL CARCINOMA (NPC) BY EPSTEIN -BARR VIRUS (EBV)
Dai Duy Ban, Pham Cong Hoat, Truong Nam Hai,
1
Nguyen Dinh Phuc
Institute of Biotechnology;
1
Institute of Otorhinoeryngology, Vietnam
In Vietnam nasopharyngeal cancer (NPC) is usually detected late when the cancer has disseminated. The treatment is,
therefore, difficult and complicated. Integration of viral DNA into the epithelial cells of nasopharyngeal tissue may
serve as a target for early diagnosis of NPC. A specific primer pair was constructed aiming to detect the presence of
EBV and was used to amplify integrated viral DNA in the biopsies of nasopharyngeal tumours of patients. 14 DNA
samples were extracted from tumour biopsies and used as templates. We detected the presence of EBV gene in all 14
cases. Comparing the results obtained with histological examination only one case did not coincide. In this case EBV
gene was positive by PCR but NPC cells were not detected.
FP4:
USE OF SPE AND HPLC FOR ISOLATION AND ANALYSIS OF MITRAGYNINE AND METABOLITES FROM URINE OF KETOM USER.
Mohd Isa Wasiman, Jalilah Hassan & Zakiah Ismail
Biochemistry Division,
Institute for Medical Research, Jalan Pahang, 50588, Kuala Lumpur
The use of ketom as heroin withdrawal suppression among drug users has been reported in the Northern part and East
Coast of Peninsular Malaysia. Recent use of ketom can be detected by detecting mitragynine and the metabolites in
urine. We described here a procedure for isolation and analysis of mitragynine and the metabolites using solid phase
extraction (SPE) and high performance liquid chromatography (HPLC). 5 ml of urine were loaded onto C18 SPE disk
column and eluted with acetonitrile. The extracts were chromatographed on C8 HPLC column with acetonitrile/water
(90:10) as mobile phase. The eluents were monitored using ultraviolet (UV) detector at 230 nm. The authenticity of
the separated compounds was determined using UV spectrum. The extraction recovery was in the range 61 - 98 %. No
other drugs or metabolites were found to interfere with the established procedure